Monday, November 1, 2010
Fluorescence in situ hybridization (FISH) is an invaluable tool in molecular ecology. Individual bacteria of targeted species are detected microscopically, located and quantified in the background of a complex population. The technique is fast and can be performed in a single afternoon. Samples from natural or engineered ecosystems and laboratory enrichment cultures are hybridized with oligonucleotide probes labeled with fluorophores. The probes target the ribosomes and depending on the probe sequence, the probes bind to a specific species, genus, family or even an entire Domain of life (such as Archaea). If appropriate controls are performed, FISH is very specific and gives unambiguous results.
source : www.microbiology.science.ru.nl